Applications

QPI of rat sarcoma cell mitosis (inverted LUT, contrast enhanced by adaptive contrast control method)Many different types of samples can be observed including adherent cells in monolayers, thin tissue sections or plant samples. The sample preparation is quite straightforward; simply requiring to pour the cell suspension into the observing chamber with no further procedures needed. The cells can be also seeded into the perfusion chamber and perfusion system and different treatments can be applied. Cell reactions to the treatment can then be observed online.

Application examples

  • CELLULAR PROCESSES
    • Cell death (apoptosis, necrosis)
    • Autophagy (autophagosomes formation)
    • Cell life cycle, cell proliferation, mitosis
    • Testing reactions of cells to a specific treatment, cytotoxicity
  • ANALYZING CELLULAR MORPHOLOGY
    • Cell dry mass evaluation
    • Changes in cell dry mass distribution, cell growth
    • Changes in cell area, perimeter, circularity
    • Multimodal imaging (QPI automatically correlated with fluorescence, DIC or brightfield)
  • CELL DYNAMICS
    • Label free time-lapse imaging (up to one week)
    • Cell motility, migration, cell tracking, directionality and speed of movement
    • Microvesicular transport
    • Cell differentiation, counting
  • CELLS IN SCATTERING MEDIA AND 3D ENVIRONMENT
    • Imaging in scattering non-transparent media (e.g. phospholipid emulsions)
    • Imaging in 3D environments (e.g. collagen extracellular matrix)

Publications:
Download this file (Balvan et al. - 2015 - Oxidative Stress Resistance in Metastatic Prostate Cancer Renewal by Self-Eating.pdf)PDF for downloadBalvan et al. - 2015 - Oxidative Stress Resistance in Metastatic Prostate Cancer Renewal by Self-Eating
Download this file (JBO_Collakova.pdf)PDF for downloadJ. Collakova, et al.: Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion, J. Biomed. Opt. 20(11), 2015.
Download this file (JBO_Krizova.pdf)PDF for downloadA. Krizova, et al.: Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior, J. Biomed. Opt. 20(11), 2015.
Download this file (PLOSONE_Balvan.pdf)PDF for downloadJ. Balvan, et al.: Multimodal holographic microscopy: distinction between apoptosis and oncosis, PloS one, 2015.