Imaging in scattering media and 3D environment is enabled by coherence-gating effect, a special feature of Q-PHASE. Nowadays imaging of cell dynamics in scattering media and 3D environments is an important part of cancer research.
Scattering media are usually opaque and imaging by common methods is not possible.
QPI of reaction of human colorectal cancer cells to treatment with 0.15% Biologically active phospholipid emulsion (scattering media)
Cancer research is an important part of modern medicine. QPI provided by TESCAN Q-PHASE microscope allows observation of cells reactions to different treatments without any other added dyes.
Presented video shows the reaction of human colorectal cancer cells to treatment by 0.15% active phospholipid emulsion for nearly 6 hours. Although active phospholipid emulsion acts as a scattering medium, Q-PHASE provides clear images of the situation. Points with higher cell mass densities are in yellow-red color and points with lower cell mass densities in blue-green color.
Courtesy of Jana Čolláková, Brno University of Technology.
Relevant publication: J. Collakova, et al.: Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion, J. Biomed. Opt. 20(11), 2015.
Cells in collagen gel
In experiments with cells in collagen gel QPI provided by TESCAN Q-PHASE microscope enables recording both the cell activity and reaction of the collagen fibers.
Moreover the visualization of cells in the simulated extracellular matrix is more precise with Q-PHASE when compared to Zernike based methods because Q-PHASE does not suffer from halo artefact. Owing to low coherence of illumination the quality of imaging is not substantially impaired by the presence of the matrix. In video human sarcoma cell produces prolonged protrusions which apparently attach to the collagen fibrils in the gel and exert traction. In used colors darker shades represent areas with higher mass density such as collagen fibrils and cell.