Intracellular processes such as movement of subcellular structures, creation of vacuoles and others can be observed in Q-PHASE with good lateral resolution and high contrast without any additional labelling.
Cell in mitotic phase
Mitosis and cytokinesis together define the mitotic phase of an animal cell cycle - the division of the mother cell into two identical daughter cells. At the beginning of the video, cell chromosomes are organized along the metaphase plate and divided into two identical sets. After that, each set of chromosomes is pulled towards opposite ends of the cell, new nuclear envelope is created and the cell is divided.
Creation of vacuoles
Creation of vacuoles in human malignant melanoma cells after treatment. Cytoplasmic vacuoles are created as a physiological response to various stimuli. Massive vacuolization of cytoplasm occurs as a reaction to cell damage leading to necrosis.
Courtesy of Tereza Renzová, Masaryk University
Movement of subcellular structures
The good lateral resolution and axial sensitivity of the technique enable observation of slight processes, such as movement of subcellular structures and secreted mass. This is advantageous in monitoring of changes in cell behavior and metabolism intensity in different environments or after treatments. Reaction of cells to environment or treatment is a fundamental principle of studies in cancer research, drug testing and toxicology.